A recent study conducted at Cornell University in New York focused on the effects of pollen from Bt corn on monarch butterfly caterpillars. The article, published in the scientific journal Nature, was widely reported in newspapers and other mass media in late May and early June 1999. This article attempts to present various views associated with this topic now that sufficient time has passed for different opinions to be expressed. This article relies heavily on information presented by Marlin Rice, Extension entomologist, Iowa State University at the following website: http://www.ipm.iastate.edu/ipm/icm/1999/6-14-1999/monarchbt.html
Bacillus thuringiensis (Bt) is a bacteria that occurs naturally in the soil. It produces an endotoxin crystal that attacks the gut membrane and creates pores, which cause leakage and swelling. The swelling continues until cells burst, which allows the gut contents to leak into the insect's blood, disrupting the blood pH and resulting in paralysis and death within 24 to 72 hours. In Bt corn, the genes in the bacteria that code for the production of this endotoxin crystal are inserted through genetic engineering into the corn plant. The resulting corn plants produce the endotoxin crystal, causing the death of caterpillars that feed on them. This toxin is produced in many locations of the corn plant, including the pollen.
The caterpillar, or larval stage, of the monarch butterfly feeds on milkweed. Because some milkweed grows next to corn, there is the potential that Bt corn pollen may drift onto milkweed and affect monarch larvae. The Cornell experiment was conducted in the laboratory with milkweed leaves that were dusted with Bt corn pollen, dusted with nonBt corn pollen, and not dusted at all. Three-day-old monarch caterpillars were then placed on the leaves and allowed to feed. After 4 days, the experiment was terminated and milkweed leaf consumption, caterpillar survival, and larval weight were recorded. The results of the study were that 44 percent of the monarch larvae died that fed on the Bt-pollen-coated leaves, and that surving larvae were less than half the size of larvae that fed on pollen-free leaves. No caterpillars died that ate leaves dusted with regular corn pollen or the control leaves.
Concerns about the study are that the amount of pollen put on the milkweed leaves is not reported, so one cannot determine how close the amount of pollen is to that occurring on milkweeds growing near cornfields. Monarch butterflies lay eggs on plants in open areas and are thus unlikely to lay eggs on milkweeds growing within cornfields.
In a study conducted at Iowa State University, potted milkweeds were placed within and at various distances from fields of Bt and nonBt corn. After 48 hours, 19 percent of the newly hatched monarch larvae in the area of Bt corn pollen died, compared to zero percent in the nonBt corn pollen treatment, and 3 percent on plants that were not placed near cornfields. This ongoing study will continue throughout this year.
Both studies suggest that some monarch caterpillars are killed when they eat Bt corn pollen. It is not known whether monarch larvae can avoid eating pollen on a milkweed in a natural environment or whether corn pollen is evenly distributed on all leaves on a milkweed. No studies have been conducted to determine the actual mortality of monarchs on milkweed near cornfields. Also, not all acres of corn are planted to Bt hybrids; estimates for 1999 suggest that 30 percent of the acres in the Corn Belt will be planted with Bt corn.
Not all Bt corn pollen may be harmful to monarchs. Fact sheets from the U.S. Environmental Protection Agency show the concentration of Bt protein in various varieties of genetically engineered Bt corn, ranging from 7.1 micrograms per gram of pollen to presumably zero (as no protein could be detected).
Monarch butterflies locate milkweed by sight and prefer to lay their eggs on small milkweed plants 3 to 18 inches in height. The female butterfly most easily finds these small plants in fencerows, ditches, and pastures, as well as on rough ground. Because corn pollen is relatively heavy, only about 30 percent of it drifts farther than 26 feet. The question that remains is how much corn pollen does it take to kill monarch caterpillars?
Previous studies have shown that the twelvespotted lady beetle, green lacewing, and insidious flower bug are not affected by corn pollen known to contain Bt. These three species of insects eat both European corn borer eggs and corn pollen. However, none are caterpillars, and the type of Bt used in Bt corn is primarily effective against caterpillars. However, a different study found that green lacewing larvae had increased mortality when they ate European corn borers that died after eating Bt corn, compared with borers that fed on nonBt corn.
If Bt corn is not used, recent studies have shown that 30.6 percent of growers use insecticides for first-generation and 15.3 percent for second-generation European corn borer. The insecticides used are either broad-spectrum ones (that kill both monarch caterpillars and many beneficial insects) or Bt (as a spray or granule that also kills monarch caterpillars whose milkweeds are hit by the insecticide). How far the insecticide extends past the cornfield when it is applied varies.
When Bt corn is planted, nonBt corn is planted nearby so that any European corn borers surviving the Bt corn and developing into moths are likely to mate with nonresistant moths. This practice greatly reduces the likelihood that the resistance will be passed on and maintained. By planting these nonBt corn refuge areas as end rows and border rows, the movement of Bt corn pollen out of fields onto nearby milkweeds would be reduced.
The monarch butterfly is not an endangered or threatened species. Its numbers are probably threatened most through loss of habitat due to increased urbanization. However, a few gardeners plant some of the more attractive milkweed species, which returns a small percent of this habitat. The other threat is the elimination areas where the butterfly overwinters. Small percentages of monarchs spend the winter in central California and in Florida. Most monarchs winter in Mexican mountain passes that are threatened with development of various types-perhaps even ecotourists that come to look at the large numbers of butterflies. Loss of wintering sites in Mexico would result in major reductions, to even total elimination of monarchs in the Midwest.
What is the bottom line? If 30 percent of growers plant Bt corn (which is likely to affect monarchs and other caterpillars near the field), but 30 percent spray nonBt corn with insecticide toxic to monarchs, other caterpillars, and other insects near the field, the situation appears to be close to a draw. Even if border and end rows are planted with nonBt corn, surveys say that 30 percent are still likely to treat those rows with an insecticide that kills caterpillars.
Additional research is needed to determine how severe the Bt corn pollen mortality is to monarchs in actual field situations. The use of Bt corn reduces insecticide use and thus reduces impact on other insects and other parts of the environment. In the meantime, it is probably possible to develop additional Bt corn varieties that do not contain Bt in the pollen, creating a better situation for monarchs and other caterpillars.
(Phil Nixon)
Globally, about 76,000 tons of methyl bromide are used each year, with North American use the highest proportion (43 percent), followed by Europe (24 percent), Asia (24 percent, includes Israel and the Mideast), with South America and Africa combined using the least (9 percent). For a detailed discussion about methyl bromide alternatives and the current science linking methyl bromide to ozone depletion, see EPA's website, http://www.epa.gov/ozone/mbr/, or call the Ozone Hot Line at 1-800-296-1996.
(Bruce E. Paulsrud)
Agricultural chemical use is expected to decrease dramatically in the coming decade, as crops genetically engineered for resistance to herbicides and insect pests garner even larger shares of the U.S. market. "We project . . . that chemical use would go down dramatically, especially herbicides," says Randy Thompson, manager of market analysis and development for Doane marketing research.
Doane recently released a third and final volume of the report, Biotech 2007: Business Analysis, which forecasts the impact of genetically engineered seed types on the market for individual chemicals used on three row crops-soybeans, corn, and cotton. In the report, line-item forecasts are made through 2007 and include 26 seed types and 128 active ingredients representing all three crops, the company said. The forecasts are based on the actual use of the pesticides on genetically enhanced seeds in 1996 and 1997, as well as on nearly 13,000 interviews with growers and 85 interviews with experts in crop biotechnology, Thompson said.
According to the report, raw total crop acres in the United States planted to soybeans, corn, and cotton are projected to increase from 165 million in 1997 to 170 million in 2007. Of these, genetically enhanced acres are projected to increase from 12 to 83 percent of the total acres planted to the three crops. Conversely, conventional acreage is projected to decline from 145 million acres in 1997 to 20 million acres in 2007, a fivefold drop. (Adapted from the University of Arizona Extension, June 1999, and P&TCN, vol. 27, no. 12.)
There is no doubt this impressive and relatively young technology will continue to change the face of agriculture. However, will over 80 percent of the U.S. corn, soybean, and cotton acres for 2007 be planted using genetically enhanced seed? That question can only be answered by domestic and foreign consumers during the intervening years. As with any new technology, plenty of concerns have been raised. The cautious and the critical commonly focus on four main issues when debating the merits of genetically modified crops.
1. Markets. If the technology substantially limits prospects for commodity exportation, or burdens the distribution system with the cost of dual marketing channels, what is the real financial gain?
2. Contamination. The potential for contamination of traditional crops through pollination and during seed and commodity transport threatens the integrity of any group marketing GMO-free commodities. Although the use of Bt corn substantially reduced the use of conventional insecticides, researchers recently raised a concern about non-pest insects that may eat the pollen produced by these plants. The use of nonselective herbicides on genetically modified crops greatly simplifies weed management but creates significant problems when misapplied, either due to poor planting records or through drift. Finally, there is concern that this technology will enable the transfer of known or unknown genes producing compounds that cause allergic reactions in some consumers.
3. Pest resistance. History tells us that any pesticide (conventional or plant-produced) or source of insect or disease resistance that is continuously overused will eventually fail. Organic growers are particularly worried that Bt crops will speed the development of insect resistance and cause Bt sprays, a valuable organic insect management tool, to become ineffective. Although many different nonselective herbicides and types of Bt endotoxins may be waiting in the wings, the extent of this diversity does have limits. No one wants to accelerate the loss of any crop protection option.
4. Agronomics. Will the use of this technology cause additional reductions in the use of other pest management practices, such as crop rotation and tillage? If so, we may have to deal with weed, insect, and disease pests that have not presented a problem in the past. On the other hand, reduced tillage may save significant amounts of topsoil.
Finally, to comply with the Food Quality Protection Act of 1996, the EPA, grower groups, and universities are working hard to get a handle on actual pesticide use, particularly for the many organophosphate (OP) insecticides. Depending on the final numbers, many OP uses may be eliminated or substantially limited in some agricultural and horticultural areas. Will biotechnology reduce the use of OPs enough to reduce the loss of OP uses? It certainly won't hurt, but I don't think anyone's crystal ball is this clear! There will be no easy answers as everyone-growers, industry, educators, consumers, and regulators-adjusts to the complexity of 21st-century crop production.
(Bruce E. Paulsrud)
New Trait/Organism |
Regulated by: |
Reviewed for: |
Insect or Viral Resistance in food crop |
USDA EPA FDA |
Safe to grow Safe for the environment Safe to eat |
Herbicide Tolerance in food crop |
USDA EPA FDA |
Safe to grow New use of companion herbicide Safe to eat |
Herbicide Tolerance in ornamental crop |
USDA EPA |
Safe to grow New use of companion herbicide |
Modified Oil content in food crop |
USDA FDA |
Safe to grow Safe to eat |
Modified flower color ornamental crop |
USDA |
Safe to grow |
Modified soil bacteria degrades pollutants |
EPA |
Safe for the environment |
Within USDA, the Animal and Plant Health Inspection Service (APHIS) is responsible for protecting U.S. agriculture from pests and diseases. Under the authority of the Federal Plant Pest Act, APHIS regulations provide procedures for obtaining a permit or for providing notification, prior to "introducing" a regulated article in the United States. Regulated articles are considered to be organisms and products (altered or produced through genetic engineering) that are plant pests or for which there is reason to believe they are plant pests. The regulations also provide for a petition process for the determination of nonregulated status. Once a determination of nonregulated status has been made, the product (as well as its offspring) no longer requires APHIS review for movement or release in the United States.
As a part of the Department of Health and Human Services, FDA (under the authority of the Federal Food, Drug, and Cosmetic Act) regulates foods and feed derived from new plant varieties. FDA policy is based on existing food law and requires that genetically engineered foods meet the same rigorous safety standards as required of all other foods. FDA's biotechnology policy treats substances intentionally added to food through genetic engineering as food additives if they are significantly different in structure, function, or amount from substances currently found in food. Many of the food crops currently being developed using biotechnology do not contain substances that are significantly different from those already in the diet and thus do not require premarket approval. Consistent with its 1992 policy, FDA expects developers to consult with the agency on safety and regulatory questions.
EPA ensures the safety of pesticides, both chemical and those produced biologically. EPA's Biopesticides and Pollution Prevention Division was created in 1994 to deal with biopesticides. Biopesticides (also known as biological pesticides) are certain types of pesticides derived from such natural materials as animals, plants, bacteria, and certain minerals. At the end of 1998, there were about 175 registered biopesticide active ingredients and 700 products. Biopesticides fall into three major classes:
Ever since it was developed, EPA's "plant-pesticide" class has been subject to debate. In late April, the U.S. EPA requested suggestions to replace the term "plant-pesticide." In response, the Council for Agricultural Science and Technology (CAST) submitted the following statement on May 21.
"CAST finds the term 'plant-expressed protectant' acceptable to replace the 'plant-pesticide' term. There may be alternative terms suggested that would be acceptable as well; however, our members would strongly object to any term that includes the word 'pesticides' in the title. Plants through their natural evolution and selection processes contain many genes for resistance to pests. In addition, plant breeders have long assisted plant survival to specific pests by crossing plants to find varieties with increased resistance. These two selection approaches are not referred to as plant-pesticides. Neither should the technology that utilizes genetic engineering result in the designation 'plant-pesticides.' The term 'plant-expressed protectant' more accurately describes the actual situation."
This statement, along with CAST's October 1998 issue paper The Proposed EPA Plant Pesticide Rule can be viewed at (http://www.cast-science.org9905epar.htm). You can order these documents by writing to Kayleen A. Niyo, Ph.D., Managing Scientific Editor, Council for Agricultural Science and Technology, 4420 W. Lincoln Way, Ames, IA 50014-3447. Phone, (515)292-2125; fax, (515)292-4512; Internet: cast@cast-science.org.
(Bruce E. Paulsrud, adapted from "Regulatory Oversight in Biotechnology" website, http://www.aphis.usda.gov/biotech/OECD/usregs.htm)